T-cell activation without proliferation in juvenile idiopathic arthritis

T-cell activation without proliferation in juvenile idiopathic arthritis

A study was done to determine if the differentiation and activation phenotype of T cells in synovial fluid (SF) from patients with juvenile idiopathic arthritis (JIA) is associated with T-cell proliferation in situ. Mononuclear cells were isolated from 44 paired samples of peripheral blood and SF. Differentiation and activation markers were determined on CD4 and CD8 T cells by flow cytometry. C...

T-cell regulation in juvenile idiopathic arthritis

Methotrexate restores effector T cell responsiveness in juvenile idiopathic arthritis

Methods Peripheral blood mononuclear cells (PBMCs) of 25 JIA patients were isolated prior to the start of MTX (T0) and 3 (T3) and 6 (T6) months after MTX start. Frequency and phenotype of FoxP3+ Tregs were analyzed ex vivo by flow cytometry and their suppressive function in CFSE suppression assays. Proliferation of CD4+ and CD8+ Teffs was determined with CFSE upon a 4-day culture in the presenc...

Multicentric Carpotarsal Osteolysis Mimicking Juvenile Idiopathic Arthritis

Background Multicentric carpotarsal osteolysis (MCTO), a skeletal dysplasia presents in early childhood mimicking juvenile idiopathic arthritis (JIA). Recognition of this syndrome is essential to avoid unnecessary treatment with immunosuppressive agents because of different course and treatment. Case Report A 3-year-old boy presented with swelling and restriction of right wrist joint and left ...

Macrophage Activation Syndrome as the First Presentation of Juvenile Idiopathic Arthritis

Macrophage activation syndrome (MAS) is a rare feature of rheumatic disorders in children and adolescence and its presentation as the first symptom of rheumatic disorders is very infrequent. A 9-year-old girl, in whom MAS developed, was admitted to our Hospital in Tehran, Iran. She suffered from high grade fever and rash followed by multiple joint swelling months afterwards. Bone marrow aspira...